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Franceschi Microscopy & Imaging Center Franceschi Microscopy & Imaging Center

Leica SP-8 Confocal Laser Scanning Microscope

This Leica TCS SP8 X is housed in room 121 of the Franceschi Microscopy and Imaging Center in Abelson Hall. It is equipped with the following:

Microscope Type:  Upright          Stage:   Fully motorized

Lenses (all corrected for coverslips):

  • 10x/0.40 Dry
  • 20x/0.75 Dry
  • 40x/1.3 Oil Immersion
  • 63x/1.4 Oil Immersion
  • 63x/1.2 Water Immersion

Excitation Laser Lines:

  • Picosecond Pulsed 405nm Diode
  • Tunable Pulsed White Light Laser 470nm to 670nm (up to 8 excitation lines can be used)

Detectors:

  • 2 Lieca HyD detectors
  • 2 PMTs
    • The confocal detectors and PMTs have adjustable band pass capabilities
  • 1 transmitted light PMT

Fluoro-Filtercubes:

  • I3: emission BP 450nm-490nm
  • N2.1: emission BP 510nm-560nm
  • A: emission BP 340nm-380nm

Transmitted Light:  Bright Field

Incident Light:   Fluorescence

Additional Features:

  • Z-Stacking
  • High Resolution Digital Image Capturing
  • FRAP (Fluorescence Recovery After Photobleaching)
  • FRET (Förster Resonance Energy Transfer)
  • FLIM (Fluorescent Lifetime Imaging Microscopy)
  • PicoHarp 300 TCSPC System and Picosecond Event Timer

 

Leica SP-5 Confocal Laser Scanning Microscope

This Leica SP5 is is housed in room 129 of the Franceschi Microscopy and Imaging Center in Abelson Hall. It is equipped with the following:

Lenses:

  • 2.5x/0.07 Dry
  • 10x/0.30 Water Immersion
  • 63x/1.20 Water Immersion
  • 20x/0.70 Oil Immersion
  • 63x/1.4 Oil Immersion

Excitation Laser Lines:

  • 405nm UV Diode
  • 458 Argon
  • 476 Argon
  • 488 Argon
  • 496 Argon
  • 514 Argon
  • 543 HeNe
  • 594 HeNe
  • 633 HeNe

Fluoro-Filtercubes:

  • GFP
  • A
  • N21
  • EMP
  • Ana

Transmitted Light:

  • Bright Field
  • Differential Interference Contrast
  • Polarized

Incident Light:  Fluorescence

Combination Light:

  • Fluorescence-Differential Interference Contrast
  • Confocal Scanning

Additional Features:

  • Resonant Scanner
  • Z-Stacking
  • High Resolution Digital Image Capturing
  • FRAP (Fluorescence Recovery After Photobleaching)
  • FRET (Fluorescence Resonance Energy Transfer)

Leica SP-5 Confocal Scanning Microscope Operation Protocol