Franceschi Microscopy & Imaging Center
Franceschi Microscopy & Imaging Center
Leica SP-8 Confocal Laser Scanning Microscope
This Leica TCS SP8 X is housed in room 121 of the Franceschi Microscopy and Imaging Center in Abelson Hall. It is equipped with the following:
Microscope Type: Upright Stage: Fully motorized
Lenses (all corrected for coverslips):
- 10x/0.40 Dry
- 20x/0.75 Dry
- 40x/1.3 Oil Immersion
- 63x/1.4 Oil Immersion
- 63x/1.2 Water Immersion
Excitation Laser Lines:
- Picosecond Pulsed 405nm Diode
- Tunable Pulsed White Light Laser 470nm to 670nm (up to 8 excitation lines can be used)
Detectors:
- 2 Lieca HyD detectors
- 2 PMTs
- The confocal detectors and PMTs have adjustable band pass capabilities
- 1 transmitted light PMT
Fluoro-Filtercubes:
- I3: emission BP 450nm-490nm
- N2.1: emission BP 510nm-560nm
- A: emission BP 340nm-380nm
Transmitted Light: Bright Field
Incident Light: Fluorescence
Additional Features:
- Z-Stacking
- High Resolution Digital Image Capturing
- FRAP (Fluorescence Recovery After Photobleaching)
- FRET (Förster Resonance Energy Transfer)
- FLIM (Fluorescent Lifetime Imaging Microscopy)
- PicoHarp 300 TCSPC System and Picosecond Event Timer
Leica SP-5 Confocal Laser Scanning Microscope
This Leica SP5 is is housed in room 129 of the Franceschi Microscopy and Imaging Center in Abelson Hall. It is equipped with the following:
Lenses:
- 2.5x/0.07 Dry
- 10x/0.30 Water Immersion
- 63x/1.20 Water Immersion
- 20x/0.70 Oil Immersion
- 63x/1.4 Oil Immersion
Excitation Laser Lines:
- 405nm UV Diode
- 458 Argon
- 476 Argon
- 488 Argon
- 496 Argon
- 514 Argon
- 543 HeNe
- 594 HeNe
- 633 HeNe
Fluoro-Filtercubes:
- GFP
- A
- N21
- EMP
- Ana
Transmitted Light:
- Bright Field
- Differential Interference Contrast
- Polarized
Incident Light: Fluorescence
Combination Light:
- Fluorescence-Differential Interference Contrast
- Confocal Scanning
Additional Features:
- Resonant Scanner
- Z-Stacking
- High Resolution Digital Image Capturing
- FRAP (Fluorescence Recovery After Photobleaching)
- FRET (Fluorescence Resonance Energy Transfer)